Essential Equipment

  1. Biosafety Cabinets (BSCs): Class II BSCs for safe handling of cell cultures and viral vectors.
  2. Incubators: CO2 incubators for maintaining cell cultures.
  3. Centrifuges: High-speed and ultracentrifuges for cell and viral vector separation.
  4. Microscopes: Inverted and fluorescence microscopes for cell observation and quality control.
  5. Cryopreservation Units: Controlled-rate freezers and liquid nitrogen storage for cell and tissue    preservation.
  6. Flow Cytometers: For cell analysis and sorting.
  7. PCR Machines: For genetic analysis and quality control.
  8. Autoclaves: For sterilizing equipment and consumables.
  9. Electroporators: For gene editing and cell transfection.
  10. Automated Cell Counters: For accurate cell quantification.

Laboratory Processes

Cell Culture

  1. Isolation and Expansion: Procedures for isolating and expanding primary cells and cell lines.
  2. Media Preparation: Protocols for preparing culture media, including the use of serum-free and xeno-free formulations.
  3. Monitoring and Maintenance: Regular monitoring of cell health and sterility.

Quality Control and Assurance

  1. Sterility Testing: Regular sterility testing of cell cultures and products.
  2. Genetic Analysis: Verification of gene editing using PCR, sequencing, or other molecular techniques.
  3. Cell Viability and Potency: Assessment of cell viability, potency, and functionality.